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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Eliminating senescent chondrogenic progenitor cells enhances chondrogenesis under intermittent hydrostatic pressure for the treatment of OA

Fig. 5

Increasing senescence burden of CPCs impaired chondrogenesis and increased IL-1β secretion under IHP. Cell groups, composed of different percentage of P1 and P10 CPCs, were tested in the in vitro IHP model for 7 days. Afterwards, cell beads were collected for analysis. a Representative Saf-O and Col 2 staining. b, c Quantitative analysis of pellet scores and Col 2 levels (n = 6 sections/pellet, N = 3 pellets per group). d ELISA assay for the IL-1β level in the supernatant of collected culture medium (N = 3 repetitions per group). a Scale bar 200 μm. b–d Values are shown as mean ± SD. NS, no significance; *P < 0.05, **P < 0.01, ***P < 0.001, one-way ANOVA with Tukey’s multiple comparisons test

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