Skip to main content
Fig. 6 | Stem Cell Research & Therapy

Fig. 6

From: Eliminating senescent chondrogenic progenitor cells enhances chondrogenesis under intermittent hydrostatic pressure for the treatment of OA

Fig. 6

Eliminating senescent CPCs by senolytics induced apoptosis and enhanced chondrogenesis in an in vitro IHP model. a Representative immunofluorescence staining for Annexin V (red), nucleus (blue) of P10 CPC treated with DQ or vehicle (DMSO) for 24 h (N = 3 repetitions per group). b Quantitative analysis of the percentage of Annexin V positive cells. c–i P10 CPCs were pretreated with DQ or vehicle and then stimulated by IHP. c, d Representative β-gal staining (c) and β-gal positive cell counting (d) of CPCs (three random fields were selected, N = 3 repetitions per group). e CCK8 assay of CPCs after various time IHP stimulation. f Representative Saf-O and Col 2 staining after IHP treatment. g, h Quantitative analysis of pellet scores (g) and relative Col 2 levels (h) (n = 6 sections/pellet, N = 3 pellets per group). i ELISA assay for the IL-1β level in the supernatant of collected culture medium (N = 3 repetitions per group). b, d Scale bar 50 μm. f Scale bar 200 μm. b, d, g–i Values are shown as mean ± SD. NS, no significance; *P < 0.05, **P < 0.01, ****P < 0.0001, two-way ANOVA with Sidak’s multiple comparisons test. e Values are shown as mean ± SD. **P < 0.01, ****P < 0.0001, two-way ANOVA with Sidak’s multiple comparisons test

Back to article page