Fig. 6From: Eliminating senescent chondrogenic progenitor cells enhances chondrogenesis under intermittent hydrostatic pressure for the treatment of OAEliminating senescent CPCs by senolytics induced apoptosis and enhanced chondrogenesis in an in vitro IHP model. a Representative immunofluorescence staining for Annexin V (red), nucleus (blue) of P10 CPC treated with DQ or vehicle (DMSO) for 24 h (N = 3 repetitions per group). b Quantitative analysis of the percentage of Annexin V positive cells. c–i P10 CPCs were pretreated with DQ or vehicle and then stimulated by IHP. c, d Representative β-gal staining (c) and β-gal positive cell counting (d) of CPCs (three random fields were selected, N = 3 repetitions per group). e CCK8 assay of CPCs after various time IHP stimulation. f Representative Saf-O and Col 2 staining after IHP treatment. g, h Quantitative analysis of pellet scores (g) and relative Col 2 levels (h) (n = 6 sections/pellet, N = 3 pellets per group). i ELISA assay for the IL-1β level in the supernatant of collected culture medium (N = 3 repetitions per group). b, d Scale bar 50 μm. f Scale bar 200 μm. b, d, g–i Values are shown as mean ± SD. NS, no significance; *P < 0.05, **P < 0.01, ****P < 0.0001, two-way ANOVA with Sidak’s multiple comparisons test. e Values are shown as mean ± SD. **P < 0.01, ****P < 0.0001, two-way ANOVA with Sidak’s multiple comparisons testBack to article page