Fig. 5

Histologic analysis of the limb muscles. Tissues were stained with a H&E staining to access necrotic (**), adipocyte infiltration (*), and normal issues; b α-SMA staining and c CD31 staining to access capillary (short arrow) and mature vessels (long arrow); and d CD31 staining under magnification × 40. e Standard qualification of the number of capillary and arterioles represented as the number of CD31-positive capillary per field (× 20) and the number of α-SMA-positive arterioles per field (× 20), respectively. Results are expressed as mean ± SEM; asterisk (*) indicates significant difference of the induced MSCs groups versus uninduced MSCs group, p < 0.05