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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: TATA box-binding protein-related factor 3 drives the mesendoderm specification of human embryonic stem cells by globally interacting with the TATA box of key mesendodermal genes

Fig. 4

Depletion of TRF3 inhibits the ME differentiation of hESCs. a Cell morphology analysis of TRF3+/+, TRF3−/−-1, and TRF3−/−-2 hESCs during the ME differentiation. Scale bar = 200 μm. b qRT-PCR analysis of ME markers during the ME differentiation of TRF3+/+, TRF3−/−-1, and TRF3−/−-2 hESCs. c Western blot analysis of EOMES, T, and GAPDH. GAPDH was used as a loading control. d Immunocytochemical staining analysis of T and EOMES at ME D2 in TRF3+/+, TRF3−/−-1, and TRF3−/−-2 hESCs. Scale bar = 25 μm. Data are presented as mean ± SEM. n = 3 each. **p < 0.01, ***p < 0.001 as indicated on the figures

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Stem Cell Research & Therapy

ISSN: 1757-6512