Fig. 5
Expression changes of integrin αV, α2, and α3 during osteogenic differentiation of hDPCs. hDPCs were incubated for 7 days with BMP-2/4, followed by treatment with ODM for additional 7 days for mineralization. a Osteogenic differentiation of hDPCs. Osteogenic and odontogenic markers (BSP, DMP-1, OC, OP, Runx2) and ligamentogenic marker (SCX) were analyzed by quantitative PCR. b, c Expression changes of integrins during osteogenic differentiation of hDPCs. Cell surface expression of integrins in the osteogenic hDPCs was analyzed by flow cytometry. Values are depicted as MFIs at the indicated culture conditions. Black, incubation in media without BMP-2/4 treatment; blue, incubation in media with BMP-2/4 treatment; green, incubation in ODMs with BMP-2/4. *, p < 0.05; **, p < 0.01; ***, p < 0.005; ns, not significant