Table 1 Summary of pluripotency-related characteristics of hiPS, eiPS, and biPS derived from fibroblasts or mesenchymal cells and miPS derived from fibroblasts
From: Generation of induced pluripotent stem cells from large domestic animals
Exogenous transcription factors | hiPS | eiPS | miPS | biPS | ||
|---|---|---|---|---|---|---|
human OSKM | Murine OSKM | Human OSKM | Murine OSKM | Murine OSKM | Human OSKM | |
Morphology | Flat | n.a.* | Flat | Dome-shaped | Dome-shaped | Dome-shaped |
1st appearance of typical colonies | 5 days | n.a.* | 3 days | 3 days | 7 days | > 30 days |
Replating | Manual | n.a.* | TrypLE | TrypLE | TrypLE | n.a.** |
AP | + | n.a.* | + | + | + | – |
EB | + | n.a.* | + | + | + | n.a.** |
Tumor formation | – | n.a.* | – | + | + | n.a.** |
LIF dependence for iPSCs generation | – | n.a.* | – | + | – | n.a.** |