Fig. 6

Enhanced anti-inflammatory efficacy of 3D-exos in cisplatin-treated mice. a Real-time PCR analysis of inflammatory cytokine mRNA levels in kidney tissues (n = 6–7). b Western blotting analysis of p-p65 in kidney tissues. c Quantification of p-p65 in kidney tissues (n = 4). d Representative immunostaining images of CD68⁺ macrophages or CD3⁺ T cells in the tubulointerstitium. Scale bar, 50 μm. e Quantification of CD68⁺ macrophages in the tubulointerstitium (n = 6). f Quantification of CD3⁺ T cells in the tubulointerstitium (n = 6). g Levels of IL-6 in the mouse serum was detected by ELISA (n = 6–7). h Levels of TNF-α in the mouse serum was detected by ELISA (n = 6–7). Data are presented as mean ± SD, *p < 0.05, **p < 0.01, ***p < 0.001 vs. Cis group, ^#p < 0.05, ^##p < 0.01