Fig. 7

Enhanced therapeutic efficacy of 3D-exos in cisplatin-treated TECs. a TECs took up 2D-exos and 3D-exos. DiI-positive (red) cells were observed under confocal microscope. Scale bar, 10 μm. b DiI-positive cells detecting by flow cytometry. Blue solid peak represents cisplatin-treated TECs internalizing DiI-labeled 2D-exos, and the green solid peak represents cisplatin-treated TECs internalizing DiI-labeled 3D-exos. c The uptake efficiency of 2D-exos and 3D-exos. d Real-time PCR analysis of inflammatory cytokine mRNA levels in TECs (n = 4). e CCK-8 assay in TECs (n = 6). Data are presented as mean ± SD, *p < 0.05, **p < 0.01, ***p < 0.001, vs. Cis group, ^#p < 0.05, ^##p < 0.01, ^###p < 0.001