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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: MiR-101-containing extracellular vesicles bind to BRD4 and enhance proliferation and migration of trophoblasts in preeclampsia

Fig. 1

HUCMSC-secreted EVs encapsulate miR-101. a Expression of CD14, CD19, CD29, CD34, CD44, CD45, CD73, CD90, and HLA-DR determined by flow cytometry. b The morphology of HUCMSCs (bar = 50 μm) observed in inverted microscope. Osteogenic, adipogenic, and cartilage differentiation was dyed with Alizarin Red S staining, Oil Red O staining, and Alcian Blue staining, respectively. c The morphology of HUCMSC-derived EV determined by TEM (100 nm). d Size distribution of the HUCMSC-exosome analyzed by NTA. e The protein levels of GM130, CD63, and TSG101 determined by immunoblotting. f The expression of miR-101 in HUCMSCs and HUCMSC-EV relative to U6 determined by RT-qPCR. *p < 0.05 vs. **p < 0.01

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Stem Cell Research & Therapy

ISSN: 1757-6512