Fig. 5

BRD4 downregulation promotes proliferation and migration of placental trophoblasts by inhibiting NF-κB/CXCL11 axis. BRD4 was overexpressed or repressed in HTR-8/SVneo cells. a Overexpression and silencing efficiency of BRD4 in HTR-8/SVneo cells determined by RT-qPCR and Western blot analysis. b Cell proliferation of HTR-8/SVneo cells after treatments determined by EDU staining (scale bar = 50 μm). c Cell migration of HTR-8/SVneo cells after treatments determined by transwell assay (scale bar = 50 μm). d Venn map of intersected genes between NF-KB-related co-expression genes retrieved from MEM and eclampsia-related genes in the GeneCards database. e Network diagram of co-expression relationships among 57 candidate genes in the Coexpedia. f Expression of NF-KB, CXCL11 IL-6, TNF-α, p65, p-IkBα, and IkBα in HTR-8/SVneo cells. g The expression of NF-KB, CXCL11 IL-6, TNF-α, p65, p-IkBα, and IkBα in HTR-8/SVneo cells in response to mimic NC, miR-101 mimic, miR-101 mimic + control, and miR-101 mimic + oe-BRD4. *p < 0.05 vs. **p < 0.01. M.NC, negative control mimic; M.miR-101, microRNA-101 mimic; I.NC, negative control inhibitor; I.miR-101, miRNA-101 inhibitor; si-BRD4, short interfering RNA target BRD4; si-NC, negative control of si-BRD4; BRD4-OE, overexpressed bromodomain-containing protein 4 plasmid