Fig. 1
From: Endothelial colony-forming cells reduced the lung injury induced by cardiopulmonary bypass in rats
Characterization of ECFCs in vitro. Mononuclear cells were collected from the peripheral blood of rats and cultured with 2% FBS EGM-2 for 21 days. The mononuclear cells presented a cobblestone appearance under a light microscope (a). The mononuclear cells were also stained with PE-labelled VEGFR2 (b), FITC-labelled CD34 (c), DiI-acetyl-labelled LDL (d) and FITC-UEA-1 (e) under a fluorescence microscope to identify the ECFCs (× 400 magnification). The cells were further analysed with a flow cytometer to confirm the ECFCs (f). The cells were stained with FITC-anti-CD14 and PE-anti-CD45 (g, h). The percentage of CD14-CD45- cells was approximately 99.8%