Fig. 2
From: Endothelial colony-forming cells reduced the lung injury induced by cardiopulmonary bypass in rats
ECFC proliferation ability and tube formation. ECFCs treated with or without L-NIO were compared. The cellular viability and proliferation of ECFCs were judged by the MTT assay. Compared with that of the normal ECFCs, the viability of the ECFCs that received L-NIO treatment was significantly decreased (a). e-NOS protein expression was detected by Western blotting to investigate the effect of L-NIO on the expression of ECFCs. Compared with that of the ECFC group, e-NOS protein expression was reduced by L-NIO treatment. Representative images and quantitative analysis (b). Effect of L-NIO on the formation of ECFC tube-like structures. The results showed that compared with the cells of the normal ECFC group, ECFC cells pre-treated with L-NIO demonstrated a marked reduction in the number of tubes that were formed. Representative images and quantitative analysis (c) (#, P<0.05)