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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Induced pluripotent stem cell-derived lung alveolar epithelial type II cells reduce damage in bleomycin-induced lung fibrosis

Fig. 1

a Schedule of the differentiation protocol from iPSC to AEC2. b Open field photographs of the iPSC during differentiation, at DE stage (day 3) and AEC2 stage (day 22). The arrows indicate typical epithelial shape cells. c RT-PCR data for DE markers of cells at day 3. d RT-PCR data for pluripotency marker OCT4 of cells at day 22. e Immunofluorescence staining for OCT4 (green) in iPSC at day 0 and iPSC-AEC2 at day 22. Nuclei were stained with DAPI (blue). DE, definitive endoderm; AFE, anterior foregut endoderm; iPSC, induced pluripotent stem cells; AEC2, alveolar type II cells

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Stem Cell Research & Therapy

ISSN: 1757-6512