Skip to main content
Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Induced pluripotent stem cell-derived lung alveolar epithelial type II cells reduce damage in bleomycin-induced lung fibrosis

Fig. 4

a Flow cytometry AMNIS Image StreamX analysis of whole lung single cell preparations. Histograms and photo of cells from flow cytometry by AMNIS Image StreamX analysis of whole lung single cell preparations to detect transplanted cells in rat lungs after 1 week transplantation. Control and control + iPSC-AEC2 groups where no labeled cells were BLM and BLM + iPSC2 groups demonstrating that no positive cells (zone R4) could be found 1 week after transplanting in the rat lung. MC_BF, Multi-Channel_Bright Field; MC_CFDA, Channel_Vybrant™ Cell Fluorescent Dye; MC, Multi-Channel. b Detection of iPSC-AEC2 engraftment after cell transplantation by fluorescence microscopy. For tracking cell purposes, iPSC-AEC2 cells were labeled with green with Vybrant™ DiO. Nuclei were stained in blue with DAPI. No green cells were observed in the transplanted groups over background/autofluorescence signal in the rat lungs (control + iPSC-AEC2 and BLM + iPSC-AEC2). Magnification × 400

Back to article page

Stem Cell Research & Therapy

ISSN: 1757-6512