Fig. 2
From: Role of CD133 in human embryonic stem cell proliferation and teratoma formation
Knockout (KO) of CD133 in WA26 hESCs by CRISPR/Cas9. a Schematic diagram of the strategy for generating a CD133 knockout hESC line by CRISPR/Cas9. b PCR amplification of the targeted region in the deletion mutants. c Colony morphology of CD133 KO and WT hESCs. Scale bar = 100 μm. d Analysis of SSEA4+ cells in CD133 KO and WT hESCs by flow cytometry. e Western blot analysis of protein expression of CD133 and pluripotency markers in CD133 KO and WT hESCs. Cell lysates were collected and analyzed by immunoblotting using the indicated antibodies. f Immunofluorescence staining of SSEA4, OCT4 and TRA-1-81 in CD133 KO, and WT hESCs. Scale bar = 20 μm. ESCs were at passage 27