Fig. 4

CD133-deficient hESCs maintain differentiation capacity into three germ layers. a Differentiation in vivo of CD133 KO and WT hESCs (passage 20) by teratoma formation test following injection into nude mice. Black arrows indicate teratomas on the back of nude mice. b Hematoxylin and eosin staining of teratoma tissues derived from CD133 KO and WT hESCs. All teratomas consist of representative derivatives of three germ layers, including epidermis (ectoderm), cartilage (mesoderm), and gland epithelium (endoderm). Scale bar = 200 μm. c Immunofluorescence of the teratomas showing markers representative of three germ layers, beta-III-tubulin (ectoderm), smooth muscle actin (SMA, mesoderm), and alpha 1-fetoprotein (AFP, endoderm). Scale bar = 10 μm