Fig. 2

HucMDEs improve glucose and lipid metabolism both in vivo and in vitro. Glycolysis-related proteins (GCK, PFK, and PK) (a), glycogen synthesis-related proteins (p-GSK3β, GSK3β) (b), and gluconeogenesis-related proteins (G-6-P, PEPCK) (c) in the livers of the indicated groups were detected by Western blotting. d PAS staining in the livers of the indicated groups. e The hepatic lipid synthesis protein, SREBP-1c, and lipolytic protein, PPARα, in the livers of the indicated groups were detected by Western blotting. Glycolysis-related proteins (GCK, PFK, PK) (f), glycogen synthesis-related proteins (p-GSK3β, GSK3β) (g), and gluconeogenesis-related proteins (G-6-P, PEPCK) (h) in L-O2 cell groups of Control + PBS, 0.25 mM PA + PBS, PA + HDEs (30 μg/ml), and PA + HucMDEs (30 μg/ml) were detected by Western blotting. i PAS staining in L-O2 cells. j The hepatic lipid synthesis protein, SREBP-1c, and lipolytic protein, PPARα, in L-O2 cells of the indicated groups were detected by Western blotting. All the results are expressed as the mean ± SD (*P < 0:05; **P < 0:01; ***P < 0.001)