Fig. 3

HucMDEs promote autophagy in the livers and L-O2 cells. a BECN1 and MAP 1LC3B protein expression levels in the livers of the indicated groups were analyzed by Western blotting. b L-O2 cells were transfected with Lenti-GFP-MAP 1LC3 and treated with PA (0.25 mM), HDEs (30 μg/ml), or HucMDEs (30 μg/ml) for 48 h (scale bar: 50 μm). c Percentages of cells with more than four GFP-LC3B dots were quantified. d TEM images showing the formation of autophagosomes (the black arrows) in L-O2 cells (scale bar 1.0 μm) and the number of autophagosomes per cell was quantified e. f BECN1 and MAP 1LC3B protein expression levels in L-O2 cells of the indicated groups were analyzed by Western blotting. All results are expressed as the mean ± SD (*P < 0:05; **P < 0:01; ***P < 0.001)