Fig. 4

Hypoxia had no effect on proliferation and apoptosis of human adipose-derived stromal cells. a ADSCs had fibroblast-like and spindle-like morphology. b Representative immunofluorescent staining of CD31, CD45, CD90, and CD105. Scale bar, 100 μm. c Representative flow cytometry of CD31 (1.22%), CD45 (1.50%), CD90 (97.0%), and CD73 (86.0%) in ADSCs. IgG was used as the isotype control. d Quantification of cytometry showed that ADSCs were CD31−/CD45−/CD73+/CD90+, n ≥ 3; **p < 0.01. e Representative Oil Red O staining of adipogenesis. f Representative Alizarin Red S staining of osteogenesis. g Cell Counting Kit-8 assay suggested no significant difference in ADSC proliferation, n = 3, p > 0.05. h Representative flow cytometric analysis of Annexin V suggested no significant difference in ADSC apoptosis