Fig. 6

The influence of shrimp miR-965 on MSLC. a shrimp miR-965 inhibited the expression of cancer stemness-associated genes. MSLC were transfected with shrimp miR-965. At 24 h after transfection, the stemness-associated gene expression levels in MSLC were examined with quantitative real-time PCR. b Shrimp miR-965 reduced the tumorsphere formation capacity of MSLC. MSLC were transfected with shrimp miR-965 (or control) for 6 h followed by evaluating the tumorsphere formation capacity at consecutive generations. c Shrimp miR-965 reduced cell viability. The cell viability was evaluated at 24 h after transfection of MSLC and non-MSLC with shrimp miR-965. d The detection of apoptosis of shrimp miR-965-transfected cells using annexin V assays. Apoptosis of MSLC and non-MSLC was examined by flow cytometry at 24 h after the transfection of shrimp miR-965. e Shrimp miR-965 induced apoptosis of MSLC via caspase activity. The MSLC and non-MSLC at 24 h after the transfection of shrimp miR-965 were subjected to the caspase 3/7 activity analysis. f Shrimp miR-965 induced ER stress gene expression. MSLC or non-MSLC were transfected with shrimp miR-965. At 24 h after transfection, the ER stress gene expression levels in cells were examined with quantitative real-time PCR. In all panels, the statistical significance between treatments was indicated with stars (**p < 0.01)