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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Hypoxia-challenged MSC-derived exosomes deliver miR-210 to attenuate post-infarction cardiac apoptosis

Fig. 2

Exosomes derived from MSCs inhibit apoptosis after myocardial infarction. a Expression levels of the apoptotic proteins BAD, BAX, BCL-2, and Cleaved-CASPASE-3 were evaluated in cardiac tissue sections from sham-operated rats and coronary-ligated rats that received either no therapy (PBS), MSC exosomes, or exosomes from MSCs treated after vessel occlusion with GW4869 immediately (N = 5 per group). Western blot analysis of protein lysates obtained from the infarct border zone. Quantitative analysis of BAD, BAX, BCL-2, and Cleaved-CASPASE-3 is shown in the lower panel. b Cell injury was determined by TUNEL assay. Fluorescence staining with vital dyes shown in blue indicates live cardiomyocytes, whereas ethidium homodimer-1 staining labels dead cells green. The percentage of cardiomyocytes death is shown in the right panels. Each experiment was repeated 3 times. *p < 0.05, **p < 0.01, ***p < 0.001 for MI vs. sham. #p < 0.05 for MI + exosome vs. MI

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