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Fig. 7 | Stem Cell Research & Therapy

Fig. 7

From: Hypoxia-challenged MSC-derived exosomes deliver miR-210 to attenuate post-infarction cardiac apoptosis

Fig. 7

Hypoxia-mediated apoptosis is associated with changes in PI3K and AKT. a Co-localization of miR-210 with exosome-specific surface marker CD81 and CD63 by the immunofluorescence. b The uptake of exosome by cardiomyocytes, and the localization of miR-210 molecule with Cy3 fluorescence in cardiomyocytes. c Dual-luciferase reporter assay showed that AIFM3 was a target of miR-210. F, Firefly luciferase; R, Renilla luciferase. d, e AIFM3, p-AKT/AKT, p-PI3K/PI3K, and p-p53/p53 levels were evaluated by Western blotting in cardiomyocytes from the indicated treatment groups ± hypoxia. Quantifications are shown in the lower panel. N = 3 independent experiments. **p < 0.01, ****p < 0.0001 for hypo vs. con. ##p < 0.01 for hypo + exo vs. hypo

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