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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Plasma membrane vesicles of human umbilical cord mesenchymal stem cells ameliorate acetaminophen-induced damage in HepG2 cells: a novel stem cell therapy

Fig. 1

Characterization of hUCMSCs and PMVs.a Microphotographs show cells of passages 1, 2, and 5 (upper panel). Passage 4 hUCMSCs were induced under adipogenic, osteogenic, and chondrogenic conditions for 2–3 weeks, and undifferentiated and differentiated of hUCMSCs were examined by Oil Red O, Alizarin Red, and Alcian Blue staining, respectively (lower panel), scale bar = 50 μm. b hUCMSCs were incubated with indicated PE-conjugated antibodies and then analyzed by flow cytometry; n = 3. An isotype-match antibody was used as a negative control. c PMVs were generated from hUCMSCs after staining with respective fluorescence-conjugated antibodies and then examined by confocal microscopy, scale bar = 10 μm. d Total RNA was harvested from hUCMSCs and PMVs. Transcripts of genes characteristic of hUCMSCs were amplified by RT-PCR and then analyzed by electrophoresis. The experiment had been repeated at least three times

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