Fig. 6

PMVs rescue HepG2 cells from APAP-induced necrotic cell death. HepG2 were treated with 90 mM APAP for 3 h. After washing, cells were harvested and re-seeded in 96-wells precoated with ECM (extracellular matrix) prepared from HTB9 cells. Mito-Tempo (MT) or PMVs from hUCMSCs (with VSV-G) were added at 4 h. PBS was used as the negative control. a Cells were stained with Annexin V-FITC (green), propidium iodide (red), and Hoechst (blue) at 48 h, and subsequently, cells in an attachment were analyzed by confocal microscopy, scale bar = 50 μm. b, c Percentage of necrotic cells and viable cell number are shown as mean ± SEM (n = 3 experiments). Bars sharing a letter in common are not significantly different (P > 0.05) (one-way ANOVA Student’s t test)