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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Pax7 as molecular switch regulating early and advanced stages of myogenic mouse ESC differentiation in teratomas

Fig. 1

Muscle tissue in Pax7+/+ and Pax7−/− ESC teratomas. a Weight of Pax7+/+ and Pax7−/− teratomas (for each genotype n = 23). b, c Representative cross sections of entire teratomas stained with Harris hematoxylin and Gomori Trichrome dye. Scale bar 500 μm. d Calculation of the area occupied by skeletal muscle tissue in teratomas (for each genotype n = 9). e, f Morphology of skeletal muscle tissue (blue) stained with Harris hematoxylin and Gomori Trichrome dye. Scale bar 100 μm. g Calculation of the area of cells expressing skeletal muscle myosin heavy chains (skMyh) (for each genotype n = 9). h, i Immunolocalization of skMyh (green) and localization of nuclei (blue). Scale bar 50 μm. j, k Inserts—magnification of myofibers expressing skMyh in teratomas. Scale bar 10 μm. White bars—values for teratomas derived from Pax7+/+ ESCs, gray bars—values for teratomas derived from Pax7−/− ESCs. Data characterized by non-normal distribution (a, d, g) was normalized. Data are presented as mean ± SD. Stars represent results of Student’s unpaired two-tailed t test: *p < 0.05; **p < 0.01; ***p < 0.001

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