Fig. 3

Selected gene expression in teratomas obtained from Pax7+/+ and Pax7−/− ESCs. a Expression of mRNAs encoding pluripotency marker (Nanog), marker of primitive streak—mesodermal cell marker (Brachyury), and markers of myogenic cells (Myod1, MyoG) (for each genotype n = 7). b Expression of mRNAs encoding embryonic myoblast markers (Pax3, Nfatc4) (for each genotype n = 7). c Expression of mRNAs encoding fetal myoblast markers (Nfix, Eno3, Mck, Mef2a, Itga7) (for each genotype n = 5 to n = 9). Expression was related to the levels observed in 13.5 d.p.c. mouse embryo (E13.5; n = 3), and normalized to mRNA encoding β-actin (Actb). d–g Western blot analysis of Pax3, MyoD, myogenin, and Mck in teratomas obtained from three Pax7+/+ ESC lines and three Pax7−/− ESC lines (for each genotype n = 3). Hsp90 level was used as a loading control. Graph represents optical density of Pax3, MyoD, Myogenin, and Mck bands compared to density of corresponding Hsp90 bands (optical density of Hsp90 was accounted as 100%, OdU optical density, arbitrary units). White bar—values for Pax7+/+ teratomas, gray bar—values for Pax7−/− teratomas. Data characterized by non-normal distribution (c, Eno3) was normalized. Data are presented as mean ± SD. Stars represent results of Student’s unpaired two-tailed t test: *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001