Fig. 5

Analysis of basal lamina and NMJ functionality in Pax7+/+ and Pax7−/− teratomas. a–c Expression of mRNAs encoding Lama4, Lama5, and Lamb2 (for each genotype n = 8 to 6). d, e Expression of mRNAs encoding neuronal markers RbFox3 and Otx2 (for each genotype n = 6 to n = 7). f Immunolocalization of neurofilament (green, left column, TL transmitted light), synaptophysin (red, middle column; BTX—green; nuclei—blue), and Fasciculin (red, right column; BTX—green; nuclei—blue) in teratoma muscles. Scale bar 10 or 100 μm, as indicated. White bars—values for teratomas obtained from Pax7+/+ ESCs, gray bars—values for teratomas obtained from Pax7−/− ESCs. Data are presented as mean ± SD. Stars symbolize results of Student’s unpaired two-tailed t test: *p < 0.05; **p < 0.01