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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Comparison of senescence-related changes between three- and two-dimensional cultured adipose-derived mesenchymal stem cells

Fig. 1

ADMSCs from the third passage were cultured in 2D and 3D cultures. a, b At 3 days and 7 days, cells were relatively homogeneous; they had characteristic spindle shapes and had smooth cell surfaces. c At 14 days, cells still maintained the characteristic MSC shape, but some cells appeared to have pseudopod-like structures and were longer and flatter. d At 21 days, cell shape was flat, and almost all ADMSCs lost their MSC shape; cells were focally aggregated and exhibited a “fried egg” morphology. eh For 3D culture, most cells were round in shape and the cell density gradually increased. g, h At 14 days and 21 days, some cells appeared to adhere to the vessel wall. il Cells re-adhered to the vessel wall after 3D culture for 3 days, 7 days, 14 days, and 21 days, respectively. The cell shape gradually became longer and flatter, but most cells maintained elongated spindle shapes and had smooth cell surfaces. Cells never lost their characteristic MSC shape. The experiment was repeated three times

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