Table 1 Primers used for RT-qPCR
Gene | Primer sequence (5′–3′) | Product size (bp) |
|---|---|---|
β-actin | Forward: CATGTACGTTGCTATCCAGGC | 250 |
Reverse: CTCCTTAATGTCACGCACGAT | ||
P16 | Forward: ATCATCAGTCACCGAAGG | 369 |
Reverse: TCAAGAGAAGCCAGTAACC | ||
P21 | Forward: CATCTTCTGCCTTAGTCTCA | 163 |
Reverse: CACTCTTAGGAACCTCTCATT | ||
P53 | Forward: CGGACGATATTGAACAATGG | 158 |
Reverse: GGAAGGGACAGAAGATGAC | ||
SOX2 | Forward: GCCGAGTGGAAACTTTTGTCG | 155 |
Reverse: GGCAGCGTGTACTTATCCTTCT | ||
OCT4 | Forward: CTGGGTTGATCCTCGGACCT | 243 |
Reverse: CCATCGGAGTTGCTCTCCA | ||
Nanog | Forward: TCTATAACTGTGGAGAGGAATC | 122 |
Reverse: GGTCTGCTGTATTACATTAAGG | ||
c-myc | Forward: GTCAAGAGGCGAACACACAAC | 162 |
Reverse: TTGGACGGACAGGATGTATGC | ||
TERT | Forward: AAATGCGGCCCCTGTTTCT | 76 |
Reverse: CAGTGCGTCTTGAGGAGCA |