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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: HGF and IL-10 expressing ALB::GFP reporter cells generated from iPSCs show robust anti-fibrotic property in acute fibrotic liver model

Fig. 2

Hepatic properties of iHep cells. a Representative picture of FACS sorted pure iHep cells. Nuclei stained with DAPI (Blue color). Bar = 200 μm. b Correct insertion of donor plasmid into the AAVS1 locus was confirmed by junction PCR. Locations of primers for junction detection are indicated (primer F (P1, P3) and primer R (P2, P4)) in Fig. 1b. c Karyotype of iHep. d Hepatocyte specific gene expression in iHep cells. RT-PCR was performed using iHep 1 and 2 lines. e Hepatogenic and anti-fibrogenic properties of iHep cells. qRT-PCR was performed using iPS, uninduced ALBpro:iPS (uHep) and induced ALBpro:iPS (iHep)

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