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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: FNDC5/irisin improves the therapeutic efficacy of bone marrow-derived mesenchymal stem cells for myocardial infarction

Fig. 1

Hypoxia exposure increased apoptosis, reduced the viability, and restrained paracrine mechanism of BM-MSCs. a Representative results of the flow cytometry analyses in BM-MSCs under hypoxia for 12 h, 24 h, and 48 h. Viable cells, Annexin V−/PI−; early apoptosis, Annexin V+/PI−; late apoptosis, V+/PI+; necrotic, V−/PI+ (scale bars, 20 μm). b, c Quantification of the apoptotic BM-MSCs. d Histogram illustrating the caspase-3 enzymatic activity in MSCsFluc+GFP+ after H/SD injury. e In vitro BLI results of BM-MSCsFluc+GFP+ under normal conditions and after HS/D injury for 12 h, 24 h, and 48 h. f Representative quantification of BLI assays. g MTT assay indicated the effects of hypoxia (0 h, 12 h, 24 h, and 48 h) on viability of MSCsFluc+GFP+. Representative ELISA assay illustrated the levels of vascular endothelial growth factor (VEGF) (h), basic fibroblast growth factor (bFGF) (i), insulin-like growth factor-1 (IGF-1) (j), and hepatocyte growth factor (HGF) (k) within MSCs under normal conditions and hypoxia for 12 h, 24 h, and 48 h. Data are expressed as the means ± SEM; n = 5; *p < 0.05

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