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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Generation of myogenic progenitor cell-derived smooth muscle cells for sphincter regeneration

Fig. 2

Characterization of MPC to MPC-SMC differentiation. a Expression of intracellular muscle-specific proteins performed by immunostainings using antibodies against aSMA, smoothelin, and desmin, each combined by nuclear staining with Hoechst dye. Representative images of cells, derived from three individual human biopsies, are shown. Scale bar (white) = 100 μm for all images. b Quantification of percentage MPC and MPC-SMC positive for myogenic markers aSMA, smoothelin, and desmin. c Fold change in cell number calculated from an initially seeded number of 500,000 cells in T175 flasks after 6 days in either growth (MPC) or smooth muscle differentiation medium (MPC-SMC). Flow cytometric quantification of d CD146 and e CD49a in populations of MPC cultivated in growth (MPC) or smooth muscle differentiation medium (MPC-SMC) for 6 days. All data (be) demonstrated as mean ± SD and derived from at least three individual human muscle biopsies. Statistical comparison performed between MPC and MPC-SMC by paired t test (ce) or two-way ANOVA with multiple testing applying Holm-Sidak correction (b) considering a p < 0.05 as statistically significant (n.s., not significant)

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