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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Generation of myogenic progenitor cell-derived smooth muscle cells for sphincter regeneration

Fig. 4

Stability of smooth muscle differentiation program of MPC-SMC. a Smooth muscle lineage marker (aSMA) expression stained by fluorescent immunostaining was compared between MPC, MPC-SMC, and MPC-SMC de-differentiated by cultivation for 3 days in growth medium following cultivation in smooth muscle differentiation medium (De-diff MPC-SMC). Representative images of MPC, MPC-SMC, and De-diff MPC-SMC derived from at least three different skeletal muscle biopsies are shown. Scale bar = 100 μm. b Formation of myotubes (red arrows) observed by fluorescence microscopy following Hoechst33342 staining for visualization of the nuclei as well as c quantification of fusion index (FI) and the number of nuclei was compared between MPC, MPC-SMC, and De-diff MPC-SMC each cultivated in skeletal muscle differentiation medium for 6 days. Scale bar = 200 μm. d Quantification of percent aSMA-positive cells in MPC-SMC and De-diff MPC-SMC was performed. e Comparison of AChE and CK enzyme activity between MPC, MPC-SMC, and MPC in skeletal muscle differentiation medium (MPC in SKDiff) and MPC-SMC in skeletal muscle differentiation medium (MPC-SMC in SKDiff). All data presented as mean ± SD of cells derived from at least three individual human muscle biopsies. Statistical analysis performed by paired t test considering p < 0.05 as significant

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