Fig. 1

Coculture of eMSCs with niche cells from menstruation and proliferative phase. a Representative image showing the distribution of colonies in monoculture, epithelial coculture and stromal coculture. Relative cloning efficiency of eMSC colonies in monoculture, coculture with epithelial or stromal cells from menstrual phase (n = 8). b The relative percentage of CD140b⁺CD146⁺ cells (n = 8). c Relative cloning efficiency of eMSC colonies in monoculture, coculture with epithelial or stromal cells from proliferative phase (n = 5). d The relative percentage of CD140b⁺CD146⁺ cells (n = 5). e Relative cloning efficiency of eMSC colonies in growth medium (ctrl), epithelial and stromal CM (n = 10). f The relative proportion of CD140b⁺CD146⁺ cells after CM treatment (n = 10). g The effect of CCM from menstrual phase on eMSCs, relative cloning efficiency of eMSC colonies in growth medium (ctrl), base media, epithelial CCM and stromal CCM (n = 5). h Relative proportion of CD140b⁺CD146⁺ cells after treatment. All the data were normalized to the control. Results shown as mean ± SEM; *P < 0.05, **P < 0.01. CCM, concentrated conditioned medium; CM, conditioned medium; eMSCs, endometrial mesenchymal stem-like cells