Fig. 2

Expression of active and total β-catenin in eMSCs after coculture with niche cells from menstruation. The protein levels of a active β-catenin and b total β-catenin in monoculture, coculture with epithelial (n = 7) or stromal cells (n = 8) relative to β-actin. Representative western blotting bands of active β-catenin, total β-catenin and β-actin in eMSCs monoculture, coculture with epithelial or stromal cells from menstrual phase. c The TCF/LEF luciferase signal of eMSCs culture in growth medium (ctrl) and epithelial CCM from menstrual phase. Data were normalized to control (n = 10). d Relative cloning efficiency of eMSCs colonies in growth medium, epithelial CCM from menstrual phase, epithelial cell CCM with DMSO and epithelial cell CCM with IWP-2 at 1.25 μM (n = 4). e Relative proportion of CD140b⁺CD146⁺ cells after treatment. Results are shown as mean ± SEM; *P < 0.05, **P < 0.01. CCM, concentrated conditioned medium; eMSCs, endometrial mesenchymal stem-like cells