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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Understanding the regulatory mechanisms of endometrial cells on activities of endometrial mesenchymal stem-like cells during menstruation

Fig. 3

Expression of LGR5 in eMSCs and endometrial stromal cells. a Immunofluorescent staining of LGR5 (red) in human endometrium. b The relative gene expression (n = 9) and c immunofluorescent images of LGR5 in eMSCs and unfractionated endometrial stromal cells. d Representative western blotting image and quantitative analysis of LGR5 expression in eMSCs and unfractionated endometrial stromal cells. (n = 6). e The TCF/LEF luciferase signal of eMSC after transfection with siRNA directed towards LGR5 (siLGR5), after transfection with scrambled control siRNA (siCtrl), culture with epithelial CCM from the menstrual phase after transfection with siCtrl and culture with epithelial CCM after transfection with siLGR5 (n = 8). Results are shown as mean ± SEM; *P < 0.05, ** P < 0.01. Scale bar, 100 μM. CCM, concentrated conditioned medium; eMSCs, endometrial mesenchymal stem-like cells; GE, glandular epithelium; LGR5, leucine-rich repeat-containing G-protein coupled receptor 5; s, stroma; siLGR5, siRNA to LGR5; siCtrl, scrambled control siRNA

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