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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Mesenchymal stem cells transfected with sFgl2 inhibit the acute rejection of heart transplantation in mice by regulating macrophage activation

Fig. 1

Construction of MSCs transfected with sFgl2-recombinant lentivirus. a The cDNA that coded for sFgl2 were cloned into the expression vector GV492. The sequence was consisted of 5′-LTR (promoter sequence) -Ubi-MCS-3FLAG-CBh-gcGFP-IRES-puromycin-3′-LTR-polyA (termination sequence). b The secretion of sFgl2 by the sFgl2-MSCs passaged to the 1st to 10th generation was detected by ELISA. c The secretions of sFgl2 by the WT-MSCs, MSCs-NC, and sFgl2-MSCs cultured with DMEM/F12 medium containing 10% FBS were detected by ELISA. d The secretions of sFgl2 by the WT-MSCs, MSCs-NC, and sFgl2-MSCs cultured with simulated inflammatory medium were detected by ELISA. The data were reported as mean ± SD, n = 3. ***Significant difference, P < 0.001

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Stem Cell Research & Therapy

ISSN: 1757-6512