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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Mesenchymal stem cells transfected with sFgl2 inhibit the acute rejection of heart transplantation in mice by regulating macrophage activation

Fig. 3

The effects of sFgl2-MSCs on the apoptosis, phagocytosis, and migration abilities of macrophages. a Flow cytometry analysis of Annexin V-FITC and PI-stained macrophages cultured alone, cultured with CsA, or co-cultured with WT-MSCs, MSCs-NC, and sFgl2-MSCs with or without the stimulation of LPS+IFN-γ respectively for 72 h. b Flow cytometry analysis of CD163 expressions of macrophages cultured alone, cultured with CsA, or co-cultured with WT-MSCs, MSCs-NC, and sFgl2-MSCs with or without the stimulation of LPS+IFN-γ respectively for 72 h. c Flow cytometry analysis of OVA-FITC-stained macrophages cultured alone, cultured with CsA, or co-cultured with WT-MSCs, MSCs-NC, and sFgl2-MSCs with or without the stimulation of LPS+IFN-γ respectively for 72 h. d Transwell migration images (scale bar = 20 μm) and their quantitative analysis of the of macrophages cultured alone, cultured with CsA, or co-cultured with WT-MSCs, MSCs-NC, and sFgl2-MSCs with or without the stimulation of LPS+IFN-γ respectively for 72 h. The data were reported as mean ± SD, n = 3. *Significant difference, P < 0.05

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