Fig. 4

The effects of sFgl2-MSCs on the AR treatment after heart transplantation. a H&E staining images (× 20 and × 200) of allografts on day 7 in all groups. b Kaplan-Meier curve performed the survival of recipients. c–e Flow cytometry analysis of CD4-FITC, CD25-APC, and Foxp3-Percp-cy5.5 (Treg) and TIGIT-PE (TIGIT⁺ Treg)-stained splenocytes isolated from the spleen samples of recipient mice was performed on day 7 in all groups and on day 14 in the CsA, WT-MSCs, MSCs-NC, and sFgl2-MSCs groups. f The expression levels of IFN-γ, TNF-α, IL-1β, IL-6, IL-12, IL-4, IL-10, and TGF-β1 in the supernatants of recipients’ serum were detected on day 7 in all groups and on day 14 in the CsA, WT-MSCs, MSCs-NC, and sFgl2-MSCs groups after heart transplantation. The data were reported as mean ± SD, n = 3. *Significant difference, P < 0.05; ***significant difference, P < 0.001