Fig. 1

Experiments in normal glucose DMEM (5.5 mM). a, b Effect of sucralose on reactive oxygen species (ROS) accumulation has been tested in vitro by Mitosox staining (n = 3). Accumulation of ROS in human mesenchymal stromal cells (MSCs) increased secondary to sucralose exposure in a dose-dependent manner. a shows florescence with Mitosox Red in presence of different concentrations of sucralose by confocal microscopy. b shows relative florescence measured from MSCs exposed to different concentrations of sucralose. c Shows MSC viability following exposure to sucralose. Viability of cells (N = 2) decreased, when exposed to sucralose in a dose-dependent manner in the presence of normal glucose concentrations in culture medium. d Effects on antioxidants and adipogenic genes in a dose-dependent manner when MSCs were exposed to sucralose in the presence of normal glucose. Statistical analysis: unpaired t test was performed by considering NG (normal glucose) as control (N = 2)