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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Long noncoding RNA LINC00314 facilitates osteogenic differentiation of adipose-derived stem cells through the hsa-miR-129-5p/GRM5 axis via the Wnt signaling pathway

Fig. 5

a Relative expression of LINC00314 in normal and induced ADSCs from week 1 to week 3. b Relative expression of LINC00314 in induced ASCs after pcDNA3.1-LINC00314 or sh-LINC00314 transfection. Relative expression of Runx2 (c), Osterix (d), and osteocalcin (e) after transfection. f Western blot analysis of Runx2, Osterix, and osteocalcin after transfection. g Alizarin red staining (21 days) and ALP staining (14 days): changes in calcium nodule formation and ALP activity after transfection. **P < 0.01, compared with the NC group; ##P < 0.01, compared with the sh-NC group

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Stem Cell Research & Therapy

ISSN: 1757-6512