Fig. 7

PTEN inhibitor SF1670 antagonized the anti-inflammatory effect of MT-Exo. RAW264.7 cells were treated with PBS, LPS (100 ng/mL), LPS + Exo, LPS + MT-Exo and LPS + MT-Exo + SF1670 for 24 h. Then, ELISA was performed to detect the concentrations of a IL-1β, b TNF-α and c IL-10 from the supernatants. The relative gene expressions of d IL-1β, e TNF-α, f IL-10, g Arg-1 and h iNOS were detected via qRT-PCR (n = 3, *p < 0.05). i The expression of PTEN, phosphorylation of AKT, AKT (total AKT) and GAPDH were also tested. GAPDH was utilized as an internal reference. j The quantification of PTEN/GAPDH and p-AKT/AKT by Western blotting (n = 3, *p < 0.05)