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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Overexpression of GATA4 enhances the antiapoptotic effect of exosomes secreted from cardiac colony-forming unit fibroblasts via miRNA221-mediated targeting of the PTEN/PI3K/AKT signaling pathway

Fig. 1

Characterization of cCFU-Fs transduced with GATA4 (cCFU-FsGATA4) and identification of exosomes from cCFU-Fs. a Immunostaining of cCFU-Fs transduced with GATA4 (cCFU-FsGATA4) or empty vector (cCFU-FsNC, control). GFP was expressed in both cCFU-FsNC and cCFU-FsGATA4. Scale bar: 50 μm. b Western blot of GATA4 in cCFU-FsGATA4 and cCFU-FsNC (n = 3). c TEM image of exosomes (n = 6). Scale bar: 200 nm. d NanoSight analysis of the particle size (n = 4). e Detection of CD9, TSG101, and Alix by Western blotting (n = 3)

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Stem Cell Research & Therapy

ISSN: 1757-6512