Fig. 2
The effect of cCFU-FsGATA4 exosomes on hypoxia-induced cardiomyocyte injury. a Cell viability of H9c2 cells after coculture with GATA4-Exo or NC-Exo under hypoxic conditions for 24 h was analyzed by CCK8 assay (n = 4, **p < 0.01 for hypoxia vs normoxia; *p < 0.05 between the indicated groups). b, c Western blot analysis of c-caspase-3 in H9c2 cells after exposure to hypoxia, GATA4-Exo, or NC-Exo (n = 3, **p < 0.01 for hypoxia vs normoxia and hypoxia vs GATA4-Exo; *p < 0.05 for GATA4-Exo vs NC-Exo). d, e Apoptotic H9c2 cells in response to hypoxia and coculture with GATA4-Exo or NC-Exo are determined by flow cytometry (Q1:viable cells, Q2: early apoptotic cells, Q3: late apoptotic cells, Q4: Necrotic cells, n = 3) (c-Cas-3: cleaved-caspase-3). (n = 3, *p < 0.05, **p < 0.01 using two-tailed Student’s t test. Data are represented as mean and SEM)