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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Correction to: MSX2 suppression through inhibition of TGFβ signaling enhances hematopoietic differentiation of human embryonic stem cells

Fig. 4

MSX2 deletion promotes upregulation of EHT signature genes. a Heatmap of hematopoietic signature genes in CD31+ cells derived from H1 WT, H1 MSX2−/− 1# and 2# cells. b GSEA of hematopoiesis-associated gene sets in CD31+ cells derived from H1 WT, H1 MSX2−/− 1# and 2# cells. c The real-time PCR analysis of RUNX1, GATA2, TAL1, and GATA1 expression in CD31+ cells derived from H1 WT, H1 MSX2−/− 1# and 2# cells at day 8 of hematopoietic differentiation. Expression is normalized to the level (= 1) of mRNA in H1 WT cells. d ChIP-qPCR analysis of MSX2-responsive elements on promoters of several EHT-associated transcription factors in H1-derived cells. Non-specific IgG was used as isotype control. All values are normalized to that of their corresponding input samples. Results are shown as means ± SD (n = 3). NS, not significant; *P < 0.05, **P < 0.01, and ***P < 0.001

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