Fig. 5
Antioxidant preconditioning promotes the cell survival of ADSCs in vitro. a Cell viability of ADSCs with or without antioxidant pretreatment in H2O2-induced cell injury model (n = 5 per group; **p < 0.01; ***p < 0.001). b Representative images of cell morphology of ADSCs treated with H2O2 (scale bar, 50 μm). c Cell apoptosis was evaluated by flow cytometry. d Western blotting of Bcl-2, Bax, and Cyclin-D1 in ADSCs treated with H2O2. e Relative expression of Bcl-2, Bax, and Cyclin-D1 in ADSCs treated with H2O2 (n = 3 per group; *p < 0.05; **p < 0.01). ADSCs, adipose tissue-derived mesenchymal stem cells; G-ADSCs, ADSCs pretreated with reduced glutathione; M-ADSCs, ADSCs pretreated with melatonin; H2O2, hydrogen peroxide