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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Extracellular vesicles produced by bone marrow mesenchymal stem cells attenuate renal fibrosis, in part by inhibiting the RhoA/ROCK pathway, in a UUO rat model

Fig. 2

Effects of BMSC-EVs on the tubular damage and collagen deposition in UUO kidneys. a BMSC-EVs protect against renal fibrosis in a dose-dependent manner. The expressions of α-SMA, E-cadherin, and Fibronectin were determined by Western blot. b BMSC-EVs protect against renal fibrosis at different treatment times. c The protein levels of α-SMA, E-cadherin, and Fibronectin were expressed as arbitrary densitometric units and normalized by the value of GAPDH. d The protein levels of α-SMA, E-cadherin, and Fibronectin were expressed as arbitrary densitometric units and normalized by the value of GAPDH. e Representative image of the HE staining from kidneys of Sham, Sham + BMSC-EVs, UUO, and UUO + BMSC-EVs rats. The bar graph depicts tubular injury scores based on HE staining. Scale bar 100 μm. f Representative image of Masson’s trichrome staining from kidneys of Sham, Sham + BMSC-EVs, UUO, and UUO + BMSC-EVs rats. The bar graph depicts renal interstitial fibrosis scores based on Masson’s trichrome staining. Scale bar 100 μm. g Representative images illustrating the infiltration of ED-1-positive macrophages of rat kidneys in different groups. Scale bar 100 μm. Quantification of the number of ED-1-postive macrophages pre-field in different groups. Scale bar 150 μm. **P < 0.01, ***P < 0.001 (compared with the Sham group); #P < 0.05, ##P < 0.01, ###P < 0.001 (compared with the UUO group)

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