Fig. 4

Effects of BMSC-EVs on the oxidative stress, cell apoptosis, renal microvasculature, and peritubular capillary pericytes in UUO kidneys. a The MDA content in the kidney was detected by commercial reagent kit. b The protein expressions for SOD1 and Catalase were detected by Western blot. c The protein levels of SOD1 and Catalase were expressed as arbitrary densitometric units and normalized by the value of GAPDH. d The protein expressions for PARP1 and cleaved caspase-3 at 7th day and 14th day after operation were detected by Western blot. e The protein levels of PARP1 and cleaved caspase-3 were expressed as arbitrary densitometric units and normalized by the value of GAPDH. f Representative images of rat kidneys of different groups by TUNEL staining. g TUNEL-positive cells counted in kidneys. h IF staining for CD34 revealed the renal microvasculature of kidneys. i IF staining for Col1α1 revealed the increase of pericytes in the UUO group. Scale bar 50 μm. *P < 0.05, **P < 0.01, ***P < 0.001 (compared with the Sham group); ^#P < 0.05, ^##P < 0.01, ^###P < 0.001 (compared with the UUO group)