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Fig. 7 | Stem Cell Research & Therapy

Fig. 7

From: Extracellular vesicles produced by bone marrow mesenchymal stem cells attenuate renal fibrosis, in part by inhibiting the RhoA/ROCK pathway, in a UUO rat model

Fig. 7

Effects of MFGE8-silenced EVs and rMFGE8 on collagen deposition, fibrosis, inflammation, and mitochondrial dysfunction in UUO kidneys. a The locations and expressions of α-SMA, E-cadherin, and Fibronectin were determined by immunohistochemical staining in the kidney sections of rats from different groups. b Semi-quantitative immunohistochemical analysis of the EMT-related protein expression in different groups. c qRT-PCR analysis of gene expression was performed for inflammatory mediators IL-1β, IL-6, TNF-α, and IL-10 at 7th and 14th day after operation. d The mRNA levels of α-SMA, E-cadherin, and Fibronectin were detected by qRT-PCR. e The protein expressions for α-SMA, E-cadherin, and Fibronectin were detected by Western blot. f The protein levels of α-SMA, E-cadherin, and Fibronectin were expressed as arbitrary densitometric units and normalized by the value of GAPDH. g Relative level of MMP in different groups. h The ATP content of kidneys in different groups. Scale bar 100 μm. *P < 0.05, **P < 0.01, ***P < 0.001 (compared with the UUO group)

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