Table 1 The sequences of forward and reverse primers
Gene | Forward sequence (5′-3′) | Reverse sequence (5′-3′) |
|---|---|---|
Col1α1 | CAGTCGATTCACCTACAGCACG | GGGATGGAGGGAGTTTACACG |
Col1α2 | GGAGGGAACGGTCCACGAT | GAGTCCGCGTATCCACAA |
Col3α1 | GTTCTAGAGGATGGCTGTACTAAACACA | TTGCCTTGCGTGTTTGATATTC |
CCL1 | CGTGTGGATACAGGATGTTGACAG | AGGAGGAGCCCATCTTTCTGTAAC |
CCL2 | CTCACCTGCTGCTACTCATTC | GCTTGAGGTGGTTGTGGAAAA |
CCL3 | ACTGCCTGCTGCTTCTCCTACA | AGGAAAATGACACCTGGCTGG |
CCL5 | TGCCCACGTCAAGGAGTATTTC | AACCCACTTCTTCTCTGGGTTG |
CCL8 | TCTACGCAGTGCTTCTTTGCC | AAGGGGGATCTTCAGCTTTAGTA |
CCL17 | ACCGCTCATCTGTGCAGACC | CGCCTGTAGTGCATAAGAGTCC |
CCL22 | AAGACAGTATCTGCTGCCAGG | GATCGGCACAGATATCTCGG |
CCR4 | GCTTGGTCACGTGGTCAGTG | GTGGTTGCGCTCCGTGTAG |
CCR8 | CAGATAATTGGTCTTCCTGCCTC | TGAGGAGGAACTCTGCGTCACA |
β-Actin | AGCTGCGTTTTACACCCTTT | AAGCCATGCCAATGTTGTCT |